The SAM specification now requires @RG tags to be included in all SAM/BAM alignments. If you are using GATK you have probably noticed that it will not run without them. Since @RG tags weren’t standard until recently, I’ve written a script to add them  in post hoc. You’ll need to install pysam and python2.7 to get it to work.

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One Response to Python: Adding Read Group (@RG) tags to BAM or SAM files

  1. Dan says:

    This is very helpful, thanks!

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